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基因抑制和激活基因的基因尺度CRISPR介导控制

Luke A Gilbert1, Max A Horlbeck1, Britt Adamson1

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA; California Institute for Quantitative Biomedical Research, San Francisco, CA 94158, USA; Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA.

Cell
|October 14, 2014
PubMed
概括

我们开发了CRISPR干扰 (CRISPRi) 和CRISPR激活 (CRISPRa) 工具,通过精确控制转录水平来系统地研究基因功能. 这些强大的方法揭示了细胞通路和基因在健康和疾病中的作用.

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