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Updated: Apr 21, 2026

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Gd(III) -PyMTA标签适用于细胞内EPR

Mian Qi1, Andreas Gross, Gunnar Jeschke

  • 1Faculty of Chemistry and Center for Molecular Materials, Bielefeld University , Universitätsstraße 25, 33615 Bielefeld, Germany.

Journal of the American Chemical Society
|October 18, 2014
PubMed
概括

这项研究表明,基于加多的旋转标签对细胞内电子偏磁共振 (EPR) 谱学是有效的. 这克服了氧化物标签的局限性,使生物大分子的细胞内结构分析成为可能.

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
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