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一个催化剂选择协议,可以从β-hairpin库中识别仿生模式

Masaomi Matsumoto¹, Stephen J Lee, Marcey L Waters

¹Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.

Journal of the American Chemical Society

|October 28, 2014

概括

研究人员开发了一种方法来选择含有histidine的,作为有机溶剂中的催化剂. 这些催化剂模仿酶活性位点,显著加速和基转移反应,速度加速至2.4 × 10^8.

科学领域:

生物化学生物化学
有机化学有机化学
催化剂是一种催化剂.

背景情况:

酶活性部位利用胺残留物催化关键的生物化学反应.
在合成系统中模仿酶催化策略是化学的一个关键挑战.

研究的目的:

开发一种选择可复制基于histidine的酶催化剂的催化剂的方法.
为了确定催化剂,加速在有机溶剂中的乙和基转移反应.

主要方法:

一个含有histidine残留物的类库的固体相合成.
在有机溶剂中选择反应性标记方案的应用.
动力分析以确定催化效率 (krel).

主要成果:

成功选择具有催化活性的含有histidine的β-hairpin.
在有机溶剂中达到高达2.4 × 10^8的速度加速 (克雷尔).
证明了选择的催化剂可以重现基于His的酶活性部位策略.

结论:

固相选择与反应性标记相结合,对于发现催化剂是有效的.
含有伊斯蒂丁的可以被设计成在非水性环境中有效催化和基转移反应.
这种方法为有机合成提供了人工酶的途径.

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