通过脂质双层和活细胞膜中的碳纳米管进行静态运输
Jia Geng1, Kyunghoon Kim2, Jianfei Zhang3
11] Biology and Biotechnology Division, Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, California 94550, USA [2] School of Natural Sciences, University of California, Merced, California 95340, USA [3] The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Nature
|October 31, 2014
概括
短碳纳米管 (CNTs) 自发地插入脂质双层和细胞膜,形成功能性的功能性膜.
科学领域:
- 生物模拟纳米技术纳米技术
- 膜生物物理学 膜生物物理学
- 合成生物学 合成生物学
背景情况:
- 开发具有高效率和选择性的合成膜通道是一个关键的挑战.
- 碳纳米管 (CNT) 由于其运输特性和孔隙结构,为膜通道平台提供了理想的性能.
- 之前的研究探讨了功能化或强制性CNT插入,但短CNT的自发运输应用仍未得到充分探索.
研究的目的:
- 为了研究短碳纳米管 (CNTs) 自发插入脂质双层和活细胞膜.
- 描述这些基于CNT的膜通道的运输特性和潜在应用,称为"CNT孔".
- 建立 CNT 体作为细胞接口,运输研究和随机传感的仿生平台.
主要方法:
- 在人造脂质双层和活细胞膜中实验性插入短CNTs.
- 电生理学测量以确定单元导电性和传输特性.
- 对离子和分子运输,导电性切换和宏分子诱导阻塞的分析.
- 调查地方收费对CNT在导电性和选择性方面的影响.
主要成果:
- 短CNT自发地插入脂质双层和活细胞膜,形成功能通道 ("CNT毛孔").
- 这些CNT体表现出单元导电性 (70-100 pS) 并运输水,质子,小离子和DNA.
- CNT 毛孔显示静态电导子态和特征性的宏分子诱导的离子电流阻塞.
- 道和膜电荷调节电导率和离子选择性.
结论:
- 简短的CNT作为有效的,在脂质双层和细胞膜中自发插入生物仿真通道 ("CNT毛孔").
- CNT 体表现出多功能运输能力和可调节的选择性,模仿生物通道.
- 这些发现确立了CNT质素作为先进细胞接口,生物运输研究和随机传感应用的有前途平台.
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