分子克隆,主要结构,以及人类生长因子可激活的Na+/H+抗载体的表达
C Sardet1, A Franchi, J Pouysségur
1Centre de Biochimie-CNRS, Université de Nice, France.
Cell
|January 27, 1989
概括
研究人员已经测序了人类对阿米洛里德敏感的-抗载体 (Na+/H+抗载体) 基因. 这种载体对于调节细胞内pH值和恢复缺陷细胞中的细胞功能至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 对阿米洛里德敏感的-抗载体 (Na+/H+抗载体) 在细胞pH稳态中起着至关重要的作用.
- 了解这种载体的分子基础对于各种生理过程至关重要.
研究的目的:
- 为了确定人类阿米洛里德敏感Na+/H+抗载体的完整cDNA序列.
- 描述Na+/H+抗载体的结构和功能.
主要方法:
- 在老鼠纤维细胞突变中的基因转移和功能补充.
- 基因组探针和cDNA库的隔离.
- DNA测序和生物信息学分析.
- 在缺陷细胞中转染cDNA的稳定表达.
主要成果:
- 确定了人类Na+/H+抗载体的完整cDNA序列.
- 预测的蛋白质的分子质量为99.354 Da,具有10个跨膜段和一个水友性细胞质尾巴.
- 稳定的cDNA表达恢复了Na+/H+抗载体功能,包括H+流入,阿米洛里德敏感性和pH调节.
结论:
- 这项研究提供了对人类阿米洛里德敏感的Na+/H+抗载体的完整序列和功能特征.
- 这些发现阐明了这种重要离子载体的分子结构和关键功能属性.
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