潜伏的内核编码成熟酶也是内核流动性所需的内核酶
J M Wenzlau1, R J Saldanha, R A Butow
1Department of Molecular Genetics, Ohio State University, Columbus 43210.
Cell
|February 10, 1989
概括
酵母线粒体内基因可以通过基因转换传播. 克斯基因中的al4α内子编码了一种蛋白质,该蛋白质同时充当成熟酶和强大的DNA内核酶,促进其自身的传播.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 在内部进化进化.
背景情况:
- 酵母线粒体内子可以编码蛋白质.
- 这些蛋白质包括成熟酶 (拼接) 和内核酶 (基因转换).
- 合1内核酶促进了内子的传播.
研究的目的:
- 为了研究克斯基因中的内子的基因转换能力.
- 为了确定al4α内子是否可以传递给其他基因.
- 描述al4α内部产物的功能.
主要方法:
- 在酵母中进行基因转换试验.
- 在DNA中检测内核分裂裂变.
- 对al4alpha开放阅读框架的分析.
主要成果:
- 组I内子,al4α,被有效地传递给缺乏它的coxl基因.
- 在受体DNA中观察到内核分解裂变,该裂变位于内核插入部位附近.
- 基因转换取决于一个完整的al4alpha开放的读取框架.
结论:
- 这种al4α内部产物既能作为潜在的成熟酶,又能作为强大的内核酶.
- 这种双重功能蛋白质参与DNA重组和内核传播.
- 双功能的蛋白质可能对内部扩散和接受至关重要.
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