一个分子规则决定了真核细胞和鞭毛的重复长度
Toshiyuki Oda1, Haruaki Yanagisawa1, Ritsu Kamiya2
1Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan.
科学家们在真核细胞的毛和鞭毛中发现了一个分子统治者. 在这些必不可少的细胞结构中,FAP59/172复合体决定了96纳米的重复长度和组件排列.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- 细胞拥有调节细胞结构大小的机制,但在真核生物中确定长度的分子基础在很大程度上是未知的.
- 假设分子统治器可以控制细胞维度,但真核生物系统中具体的例子很少.
研究的目的:
- 识别和描述基底的分子机制长度确定在真核细胞和鞭毛.
- 调查特定蛋白质复合体在建立轴突体内的重复结构单元中的作用.
主要方法:
- 使用冷电子断层扫描技术可视化了 Chlamydomonas flagella 中蛋白质复合体的高分辨率结构.
- 生物化学和遗传学方法被用来评估在轴突组合和长度调节中识别的蛋白质复合体的功能.
主要成果:
- FAP59和FAP172蛋白质在Chlamydomonas flagella中形成一个长96纳米 (nm) 的复合体.
- 删除FAP59/172复合体破坏了轴突膜结构的特征性的96nm重复.
- 设计一个长长的FAP59/172复合体导致延长的重复长度 (高达128 nm) 和改变了轴突膜组件组织.
结论:
- FAP59/172复合体作为分子统治器起作用,在乳毛和鞭毛中定义96nm的重复长度.
- 这种复合体对于轴内组件的精确排列至关重要,确保正确的结构和功能.
更多相关视频
10:01Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans
Published on: August 4, 2016
08:22A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene
Published on: September 16, 2019
相关概念视频
Replication in Eukaryotes
Replication in Eukaryotes
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Microtubules in Cell Motility
The Replisome
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Mechanism of Ciliary Motion
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
