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合成生物学. 合成生物学. 基因组编码的模拟存储器具有精确的体内DNA写入活细胞群中的DNA写入

Fahim Farzadfard¹, Timothy K Lu²

¹Synthetic Biology Group, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue, Cambridge, MA 02139, USA. MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA 02139, USA. MIT Microbiology Program, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Science (New York, N.Y.)

|November 15, 2014

概括

这项研究引入了一种新的细胞记忆系统,使用DNA作为基因组"录音机". 它可以对活细胞中的长期事件历史进行模拟记录,用于各种应用.

科学领域:

合成生物学合成生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

细胞记忆对于生物过程和合成生物学至关重要.
目前使用表观遗传开关或重组酶的方法在可扩展性和记录能力方面存在局限性.

研究的目的:

开发一种用于对活细胞群体中长期事件历史的模拟和分布式记录的新平台.
用细胞DNA作为基因组"磁带记录器"来增强数据存储.

主要方法:

在生物体中产生单链DNA (ssDNA),以响应转录信号.
与重组酶共同表达ssDNA以准特定的基因组DNA地址.
在基于输入信号的细胞群中积累精确的突变.

主要成果:

展示了细胞内ssDNA生成和向基因组突变的平台.
展示了对事件历史的模拟和分布式记录的潜力.
通过DNA修饰建立了一种通过DNA修饰创建遗传细胞记忆的方法.

结论:

开发的平台为蜂存储器提供了可扩展和高容量的解决方案.
这项技术在环境监测,生物医学诊断和先进的基因组工程方面具有潜在的应用.
基因组磁带记录器系统为复杂的生物状态机器铺平了道路.

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