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拓关联域是复制定时调节的稳定单位

Benjamin D Pope¹, Tyrone Ryba², Vishnu Dileep¹

¹Department of Biological Science, 319 Stadium Drive, Florida State University, Tallahassee, Florida 32306, USA.

|November 21, 2014

概括

复制域边界与拓关联域 (TAD) 边界对齐,协调染色体结构和复制时间. 这揭示了细胞特异复制时间如何塑造大规模基因组组织.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

细胞染色体遵循的是细胞类型特定的复制时间计划.
复制域和拓关联域 (TAD) 是关键的结构单元,但它们与复制时间的关系尚不清楚.
之前的研究表明,复制时间,染色体区和叶片相关域 (LAD) 之间存在相关性,但缺乏统一的模型.

研究的目的:

精确地绘制复制域边界,并在多种细胞类型中调查它们与TAD边界的关系.
为了使观察到的细胞类型特定的复制时间与稳定的基因组结构相协调.
开发一个大规模染色体结构和功能的统一模型.

主要方法:

在复制-定时过渡边界的复制域边界的定位.
在18种人类和13种小鼠细胞类型中映射复制域边界.
高分辨率染色体构造捕获 (Hi-C) 分析以识别TAD和染色体部分.

主要成果:

复制域边界与TAD边界有很强的相关性.
TADs的细胞类型特定的复制时间创造了大规模的亚核区.
复制-定时过渡类似于染色质和LAD协会中的晚复制区域.

结论:

提出了一个统一的模型,协调复制时间,TAD和亚核分区.
复制域边界在很大程度上是由TAD边界定义的.
细胞类型特定的复制时间影响了基因组组织和染色质特性.

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