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人类和C. elegans中的各种子基质

Agnieszka Rybak-Wolf¹, Marvin Jens¹, Yasuhiro Murakawa²

¹Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.

|November 24, 2014

概括

研究人员在转录中绘制了Dicer结合部位的地图,揭示了新的microRNA目标和非正规的角色. 大多数mRNA和lncRNA的部位都会稳定表达,并可以隔离Dicer,从而影响microRNA水平.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生物化学

背景情况:

核糖核酶Dicer对于真核小RNA和microRNA生物生成至关重要.
缺乏对Dicer目标转录的直接映射,限制了对其调节作用的理解.

研究的目的:

在人类细胞和C. elegans的转录组中识别和绘制Dicer结合部位.
为了描述Dicer-bound转录的性质和功能,超出了正规的微RNA处理.

主要方法:

对人类细胞和C. elegans.生物化学方法的应用.
对Dicer结合部位的全转录组映射.
对Dicer目标特征和相关的细胞功能进行分析.

主要成果:

数以千计的Dicer结合部位被确定,包括已知的和新的microRNA前体.
结构RNA,促进RNA和线粒体转录被确定为Dicer的目标.
大多数Dicer结合部位都在mRNAs/lncRNAs上发现,通常在完整的针头内,稳定表达并可能隔离Dicer,从而降低microRNA水平.
具有这些"被动"Dicer结合位点的mRNA与处理体/颗粒功能有关.

结论:

这项研究提供了第一个全面的Dicer目标的转录组全方位地图.
建议保留Dicer结合模式和功能在正规微RNA通路之外.
这些发现强调了Dicer在小RNA生物发生之外的调节作用,通过非正规相互作用影响mRNA稳定性和microRNA表达.

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