AF9 YEATS域将基因素乙化与DOT1L介导的H3K79甲基化联系起来
Yuanyuan Li1, Hong Wen2, Yuanxin Xi3
1Collaborative Innovation Center for Biotherapy, MOE Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Cell
|November 24, 2014
概括
AF9 YEATS域识别了基因素乙化,特别是H3K9ac. 这一发现揭示了一种新型的乙氨酸读取器,该读取器对于将基因素乙化与基因转录调节联系至关重要.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 结构生物学 结构生物学
背景情况:
- 基因基因基因表达的调节是基因基因的调节.
- 与甲基化相比,很少有蛋白质模块能够识别组 histone 的乙化.
- 了解乙氨酸读者对于破译基因调节至关重要.
研究的目的:
- 为了识别能够识别基因素乙化的新型蛋白质模块.
- 阐明由AF9 YEATS域识别乙氨酸的结构基础.
- 为了研究AF9在将基因素乙化与基因转录联系起来的功能性作用.
主要方法:
- 蛋白质 - 配体结合测试以评估AF9 YEATS域与乙化组织素的相互作用.
- 进行X射线晶体学以确定AF9 YEATS域的结构.
- 染色体免疫沉测序 (ChIP-seq) 以在全基因组范围内绘制AF9和H3K9乙化图.
主要成果:
- AF9 YEATS域强烈地与H3K9乙化结合,并且在较小程度上与H3K27和H3K18乙化结合.
- 结构研究揭示了一种新的"三明治化"子机制,用于AF9 YEATS域的乙氨酸读数.
- 观察到AF9和H3K9乙化的全基因组局部化,影响DOT1L招募和H3K79甲基化.
结论:
- YEATS域是一个新的乙氨酸结合模块.
- AF9将基因组乙化 (H3K9ac) 与DOT1L介导的H3K79甲基化联系起来.
- 这为转录控制机制提供了新的见解.
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