cdc2 激酶是一种核蛋白,对哺乳动物细胞的线粒分裂至关重要
K Riabowol1, G Draetta, L Brizuela
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|May 5, 1989
概括
这项研究表明cdc2蛋白激酶 (p34) 对于哺乳动物细胞的细胞分裂至关重要. 阻断它的功能会阻止细胞分裂,强调它在细胞循环中的关键作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- cdc2蛋白激酶 (p34) 是裂变酵母cdc2的同类物,也是Xenopus中M相促进因子的一个组成部分.
- 人类HeLa细胞同源激酶 (p34) 在线粒分裂过程中表现出最大的活性,这表明它在哺乳动物体细胞线粒分裂中发挥了作用.
研究的目的:
- 研究p34及其相关蛋白p13suc1在哺乳动物细胞分裂中的作用.
- 为了确定p34和p13suc1在哺乳动物细胞细胞周期中的局部.
主要方法:
- 微注射抗p34和抗p13suc1抗体到血清刺激的老鼠纤维细胞中.
- 对DNA合成和细胞分裂的观察.
- 免疫光显微镜以确定蛋白质定位.
主要成果:
- 微注射抗p34抗体阻断了细胞分裂,但没有影响DNA合成.
- 注射抗p13suc1抗体并没有阻断线粒分裂,但引起了异常,包括微核的形成.
- 在介相期间,p34定位在细胞核中,在线粒分裂期间,中心细胞在细胞核中.
- p13分布在细胞核和细胞质之间.
结论:
- 在哺乳动物细胞中,cdc2蛋白激酶 (p34) 对于线粒分裂至关重要.
- p34作为核和细胞中心蛋白质起作用,是细胞正常分裂所需的.
- p13suc1在调节线粒分裂方面发挥作用,但它缺少导致异常,而不是完全阻塞.
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Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
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