特定转录的高度局部化轨迹在相间核内,通过in situ杂交可视化
J B Lawrence1, R H Singer, L M Marselle
1Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.
Cell
|May 5, 1989
概括
研究人员发现特定的病毒RNA在埃普斯坦-巴尔病毒 (EBV) 感染细胞的细胞核中形成明显的"轨迹". 这一发现为在病毒延迟期间的核组织和基因表达提供了新的见解.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 潜在的爱斯坦-巴尔病毒 (EBV) 感染涉及宿主细胞核内的复杂相互作用.
- 了解病毒RNA的空间组织和运输对于破译EBV生命周期至关重要.
研究的目的:
- 研究EBV潜伏期间特定病毒RNA转录的核定位和组织.
- 探索感染细胞中RNA转录,运输和核结构之间的关系.
主要方法:
- 采用了优化用于核RNA的光检测而没有变性化的in-situ杂交.
- 采用RNAase敏感性,actinomycin D抑制和序列特异性来确认RNA检测.
- 检查了各种病毒序列,细胞系和转染序列,以评估普遍性.
主要成果:
- 在EBV感染细胞内的独特的曲线核"轨迹"中确定了特定病毒RNA的突出定位.
- 证明这些RNA轨迹从内部基因组向核外围延伸.
- 观察到其他病毒序列和非病毒转录的类似局部核RNA模式.
结论:
- 核RNA轨迹表明RNA局部化和潜在的运输与转录相结合的高度组织的系统.
- 这些发现为核组织和病毒延迟期间基因表达的调节提供了新的见解.
- 观察到的现象可能是管理特定核转录的一般机制.
相关概念视频
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...