爱斯坦-巴尔病毒小RNA (EBER) 基因:结合RNA聚合酶II和III促进元素的独特转录单元
1Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, New Haven, Connecticut 06510.
Cell
|June 2, 1989
概括
爱斯坦-巴尔病毒编码的小RNA (EBER) 基因利用独特的促进元件,包括ATF和Sp1结合点,进行高水平的表达. 这些发现揭示了EBV转化细胞中RNA聚合酶III转录的新型调节机制.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 基因法规 基因法规
背景情况:
- 埃普斯坦-巴尔病毒编码的小RNAs (EBERs) 是感染细胞中丰富的转录.
- 它们精确的转录调节和促进元素仍然不完全理解.
- EBERs代表了一个独特的RNA聚合酶III转录的类.
研究的目的:
- 阐明控制EBER基因表达的促进子元素和转录因子.
- 了解EBER在EBV转化淋巴细胞中高水平表达背后的机制.
主要方法:
- 分析EBER基因促进区域,包括基内控制区域 (A和B框) 和上游元素.
- DNAase I 足迹测定用于识别蛋白质-DNA 相互作用.
- 奥利冈核酸竞争测试以确认转录因子结合.
主要成果:
- EBER基因具有功能性的A和B盒内基因控制区域.
- 确定了三种上游元素,可以刺激体内表达的50倍.
- DNAase I 足迹检测揭示了激活转录因子 (ATF) 或相关蛋白质与核酸 -40 至 -55.5 的结合.
- Sp1蛋白或与之相关的蛋白与核酸结合 -56至 -77.
- 在 -23 和 -28 位置之间的元素类似于 TATA 盒子.
结论:
- EBERs是不寻常的RNA聚合酶III转录,由不同的促进元素调节.
- 类似ATF和Sp1的转录因子在控制EBER表达方面发挥着至关重要的作用.
- 这些调控机制可能有助于EBER在EBV转化细胞中的高丰度.
相关概念视频
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Transcription Initiation
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...


