相关实验视频

Updated: Apr 20, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

通过结合质谱和外基因组测序来预测免疫性瘤突变

Mahesh Yadav¹, Suchit Jhunjhunwala¹, Qui T Phung¹

¹Genentech, South San Francisco, California 94080, USA.

|November 28, 2014

概括

科学家们开发了一种新方法来发现瘤中的免疫原突变. 这种方法简化了针对个性化癌症疫苗的新抗原的识别和监测T细胞反应.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物信息学是一种生物信息学.

背景情况:

人类瘤积累了许多体质突变.
在主要基因相容性复合物I类 (MHCI) 分子上呈现的突变可以作为新抗原,可能引起抗瘤T细胞反应.
发现这些新抗原是具有挑战性的,因为艰苦的选方法.

研究的目的:

为了简化免疫原突变的发现.
描述免疫原突变的一般性质.
开发一种用于识别新抗原的预测算法.

主要方法:

结合全外体和转录组测序与质谱学,以识别小鼠瘤模型中的新上位素.
结合MHCI的突变的结构建模.
通过预测T细胞受体可访问的溶剂暴露突变来评估免疫性.
接种了预测免疫性的疫苗的小鼠.

主要成果:

确定了超过1300个氨基酸变化,预计约13%的氨基酸会结合MHCI.
通过质谱测量证实了预测的子集.
结构模拟的与MHCI结合,识别溶剂暴露的突变作为潜在的免疫原体.
用预测的疫苗在小鼠中引起了治疗性活跃的T细胞反应.

结论:

一个预测算法可以有效地识别免疫性突变.
这种方法有助于开发个性化癌症疫苗.
该方法允许药理动力学监测T细胞对癌症疫苗的反应.

更多相关视频

Predictive Immune Modeling of Solid Tumors

Predictive Immune Modeling of Solid Tumors

Published on: February 25, 2020

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...