基因组编辑 基因组编辑 基因组工程的新前沿是CRISPR-Cas9
Jennifer A Doudna1, Emmanuelle Charpentier2
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Department of Chemistry, University of California, Berkeley, CA 94720, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. doudna@berkeley.edu emmanuelle.charpentier@helmholtz-hzi.de.
Science (New York, N.Y.)
|November 29, 2014
概括
克里斯普尔-Cas9系统使各种生物体的基因组工程变得容易,从而彻底改变了生物研究和生物技术. 这种强大的工具促进了基因改造,为发现和治疗开发开辟了新的途径.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物技术是生物技术.
背景情况:
- 克里斯普尔 (集群定期间隔的平行体重复) 生物学已经显著发展.
- 细菌RNA引导的CRISPR-Cas9系统是基因组工程的一个关键工具.
研究的目的:
- 审查CRISPR-Cas9.9的历史和机制.
- 突出其对生物研究和生物技术的变革性影响.
主要方法:
- 关于CRISPR生物学发现的回顾.
- 阐明了CRISPR-Cas9酶机制.
主要成果:
- 克里斯普尔-Cas9在生命领域实现了简单的基因组工程.
- 基因组操纵不再是实验的瓶.
结论:
- 克里斯普尔-Cas9开启了生物发现的新时代.
- 应用范围涵盖生物技术和人类治疗.
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