在Streptococcus pneumoniae中,MapZ标记了分裂部位和FtsZ环的位置
Aurore Fleurie1, Christian Lesterlin2, Sylvie Manuse1
1Bases Moléculaires et Structurales des Systèmes Infectieux, IBCP, Université Lyon 1, CNRS, UMR 5086, Lyon, 69007, France.
Nature
|December 4, 2014
概括
一种新的蛋白质MapZ (中细胞定蛋白Z),在Streptococcus pneumoniae中FtsZ环组装之前充当分裂部位信标. 这种由酸化调节的新机制指导细菌细胞分裂.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 准确的细胞分裂需要精确地放置分裂机器.
- 在细菌中,FtsZ环聚合通常启动分裂部位识别.
- 肺炎性链球菌缺乏正规的FtsZ环定位系统,因此其分裂机制尚不清楚.
研究的目的:
- 鉴定一种新型因子,该因子参与了Streptococcus pneumoniae.分裂部位的定位.
- 阐明分裂机器在这种病原体中定位的机制.
- 研究MapZ的作用及其对细菌细胞运动的调节.
主要方法:
- 蛋白质局部化研究可视化MapZ和FtsZ动态.
- 基因操纵来评估MapZ和StkP的功能.
- 生物化学试验用于研究蛋白质-蛋白质相互作用和酸化效应.
主要成果:
- 在细胞赤道上,MapZ形成稳定的环,先于FtsZ环形成.
- MapZ与FtsZ直接相互作用,指导其定位并负面调节其组装.
- MapZ被StkP酸化,酸化和非酸化形式对Z环动态至关重要.
结论:
- 在S. pneumoniae中发现了一种涉及MapZ作为分裂地点信标的新型细胞分裂机制.
- 以MapZ为媒介的划分地点选择和FtsZ环状定位代表了一种独特的监管策略.
- 通过StkP对MapZ的酸化对于适当的细菌细胞动力学是必不可少的,突出了细胞分裂的各种进化策略.
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