玉米Spm可转移元素的基本大转录是由替代拼接生成的
P Masson1, G Rutherford, J A Banks
1Department of Embryology Carnegie Institution of Washington Baltimore, Maryland 21210.
Cell
|August 25, 1989
概括
确定了新的抑制器-突变器 (Spm) 可移植元素基因. 在Spm基因内的内tron序列编码了必需产品,并参与了新的转化调节,影响了植物中的基因活性.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 植物科学 植物科学
背景情况:
- 抑制器-突变器 (Spm) 是一个可转换元素的类.
- 了解SPM基因功能对于植物遗传学至关重要.
研究的目的:
- 为了确定新的抑制器-突变器 (Spm) 可转移元素基因.
- 调查内序列在SPM基因活动和调节中的作用.
主要方法:
- 在体外突变的突变发生.
- 克隆cDNA克隆cDNA克隆
- 对转基因烟草的分析.
主要成果:
- 在tnpA基因的内基突变显著降低了spm活动.
- 识别了含有外子和内子序列的替代拼接转录 (tnpB,tnpC,tnpD).
- 在前子1中发现了两个替代拼接捐赠点.
结论:
- 在SPM内序列中,编码了基本的基因产物.
- 斯普姆利用替代拼接来产生多样化的转录.
- 一个新的转化调节模式是由exon 1结构提出的.
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