增强剂-核心-促进剂特异性分离了发育和家庭基因调节的基因调节
Muhammad A Zabidi1, Cosmas D Arnold1, Katharina Schernhuber1
1Research Institute of Molecular Pathology IMP, Vienna Biocenter VBC, Dr Bohr-Gasse 7, 1030 Vienna, Austria.
Nature
|December 18, 2014
概括
无处不在的活性增强剂和发育特异性增强剂表现出不同的核心促进剂特异性. 这一发现将整个基因组中的家庭管理和发育基因的基因调节分开.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
背景情况:
- 基因转录依赖于RNA聚合酶II,核心促进剂和增强剂.
- 无处不在的家政基因与细胞类型特定的发育基因的调节仍然不清楚.
- 无处不在的活性增强剂的存在及其调节机制尚未得到充分理解.
研究的目的:
- 调查不同核心促进者的增强剂特异性.
- 为了确定不同的增强剂类是否调节家庭和发育基因.
- 为了识别分离这两个基因调节程序的机制.
主要方法:
- 使用了Drosophila melanogaster S2和卵巢体细胞 (OSC).
- 从核糖体蛋白 (家政) 和转录因子 (发育) 基因中分析了对核心促进者的增强特异性.
- 确认了额外的核心促进者的特异性.
- 检查了基因组分布,邻近的基因功能和核心促进元素.
- 确定了转录因子Dref和Trl.
主要成果:
- 数以千计的增强剂对家庭或发育核心促进剂表现出特异性.
- 家政增强剂在两种细胞类型中都活跃;发育增强剂显示细胞类型特异性.
- 增强剂类在基因组位置,相关的基因功能和促进元素上有所不同.
- Dref 和 Trl 分别被确定为家政和发育增强剂的关键转录因子.
结论:
- 一个编码序列的增强剂-核心促进剂特异性机制将家庭管理和发育基因调节分开.
- 这种特异性适用于成千上万的增强剂及其全基因组目标基因.
- 为了解控制基因表达的不同监管程序提供了一个框架.
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