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相关概念视频

Exon Recombination02:32

Exon Recombination

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Organization of Genes02:07

Organization of Genes

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Overview
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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相关实验视频

Updated: Apr 19, 2026

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans
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Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

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微显子会变得很大,变得很大.

Li Yang1, Ling-Ling Chen2

  • 1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology; CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Cell
|December 20, 2014
PubMed
概括
此摘要是机器生成的。

已经确定了数百个微子子,这些微子在分析中经常被遗漏. 它们的替代拼接由RNA结合蛋白调节,影响神经发生,并与自闭症谱系障碍有关.

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An Integrated Approach for Microprotein Identification and Sequence Analysis
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An Integrated Approach for Microprotein Identification and Sequence Analysis

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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相关实验视频

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Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

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An Integrated Approach for Microprotein Identification and Sequence Analysis
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 神经科学是一个神经科学.

背景情况:

  • 微显子是短的显子,在转录组研究中经常被忽视.
  • 最近的研究强调了它们的显著存在和功能相关性.

研究的目的:

  • 为了识别和表征新的微子.
  • 研究微外子在神经发生过程中的调节机制和功能影响.
  • 探讨微电子调节错误与自闭症谱系障碍之间的联系.

主要方法:

  • 使用RNA测序进行转录组分析.
  • 微微子的生物信息识别.
  • 替代拼接事件的实验验证.
  • 在神经元模型中的功能研究.

主要成果:

  • 识别了数百个以前未被描述的微子.
  • 证明特定微子子的替代拼接是由神经元RNA结合蛋白调节的.
  • 证据表明,这些微外显子在神经发生过程中调节蛋白质功能.
  • 微埃克森误调与自闭症谱系障碍的关联.

结论:

  • 微埃克森是转录组的一个重要但被低估的组成部分.
  • 它们的受调节的替代拼接在神经元发育中起着至关重要的作用.
  • 微微子的调节失调与自闭症谱系障碍的病理生理学有关.