内心炎病原体通过激活血小板诱导血管内中性粒细胞外细胞陷,促进植被形成
Chiau-Jing Jung1, Chiou-Yueh Yeh1, Ron-Bin Hsu1
1From the Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan (C.-J.J., J.-S.C.); Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Taiwan (C.-Y.Y., J.-S.C.); Department of Surgery, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan (R.-B.H.); Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan (C.-M.L.); and Department of Forensic Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan (C.-T.S.).
中性粒细胞外细胞陷 (NETs) 相互连接并捕获细菌-血小板聚合物,促进内心炎中的植被生长. DNase I 处理减少了 NET 和细菌负载,突出了 NET.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 心血管研究研究心血管研究
背景情况:
- 在内心炎期间,链球菌在心脏门上形成生物膜与血小板.
- 在植被中介于细菌-血小板聚合物被困的宿主因素是未知的.
研究的目的:
- 阐明宿主因子在内心炎中的细菌植被形成中的作用.
- 为了研究细菌-血小板聚合物捕获的机制.
主要方法:
- 使用了一种Streptococcus mutans内心炎鼠标模型.
- 研究了中性细胞外细胞陷 (NETs) 在植被发育中的作用.
- 评估了血管内DNase I预防的影响.
主要成果:
- 确定了网络,在植被中相互连接和捕获细菌-血小板聚合物.
- DNase I 治疗显著减少了植被大小和细菌殖民.
- 活性血小板和特定的IgG吸收细菌通过多个途径诱导了NET的形成.
- 细菌介导的信号通路 (Syk,Src,PI3K,p38 MAPK) 调节了血小板P-选择素的升高,并诱导了中性粒细胞的ROS依赖性素化.
结论:
- 中性细胞外细胞陷对于促进和扩大内心炎中的植被形成至关重要.
- NETs充当支架,增强细菌-血小板聚合物的被困在受伤的心脏门上的作用.
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