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Updated: Apr 18, 2026

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

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用于机械敏感膜探针的光.

Marta Dal Molin1, Quentin Verolet, Adai Colom

  • 1School of Chemistry and Biochemistry, National Centre of Competence in Research (NCCR) Chemical Biology, University of Geneva , Geneva, Switzerland.

Journal of the American Chemical Society
|January 14, 2015
PubMed
概括

新的"光"使机械敏感探头能够对脂质双层进行检测. 这些探测器具有高灵敏度和亮度,允许在生物膜和细胞中进行详细的成像.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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