使用空心碳纳米球作为光诱导的自由基发生器来克服化疗耐药性
Liming Wang1, Qiang Sun, Xin Wang
1CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China and Institute of High Energy Physics, Chinese Academy of Sciences , Beijing China.
Journal of the American Chemical Society
|January 20, 2015
概括
新型空心碳纳米圈 (HCSs) 提供多克索鲁比 (DOX),并通过近红外激光照射产生自由基. 这种方法通过增强药物输送和抑制耐药基因,打击乳腺癌细胞中的化疗耐药性.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 癌症研究 癌症研究
背景情况:
- 癌细胞的化疗耐药性,通常是由于低氧化还原状态,导致治疗失败.
- 在现场产生高度的自由基是一种有希望的战略,以克服耐药性.
研究的目的:
- 开发一种使用空心碳纳米圈 (HCSs) 的新型平台,用于共同输送多克索鲁比 (DOX) 和生成活性氧物种 (ROS).
- 研究光热疗法,增强ROS生成和化疗在克服乳腺癌细胞中抗药性的协同效应.
主要方法:
- 分散空洞碳纳米圈 (HCSs) 被合成并装载了多克索鲁比 (DOX@HCSs).
- 接近红外线 (NIR) 激光照射被用来触发DOX释放并诱导来自HCS的ROS生成.
- DOX@HCS与NIR照射相结合的疗效在耐药性人类乳腺癌细胞 (MCF-7/ADR) 上进行了评估.
主要成果:
- 被辐射的HCSs产生了持久的自由基,导致热冲击因子-1 (HSF-1) 蛋白质同位素的增加和抵抗基因的抑制.
- 尼尔射线激光照射促进了 lysosomal DOX 释放到细胞质中,促进了核进入,并提高了化疗的疗效.
- DOX@HCSs表现出协同作用,通过光热效应,ROS生成和化疗,显著降低了MCF-7/ADR细胞中的多克索鲁比抗性.
结论:
- 空心碳纳米圈 (HCSs) 提供了一个多功能平台,用于联合输送化疗药物并产生ROS来对抗抗药性癌症.
- 这种结合治疗策略,利用HCS和NIR激光照射,为治疗耐药乳腺癌提供了一个有前途的方法.
- 这些发现为克服癌症治疗中的化疗耐药性提供了新的临床策略和见解.
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