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重新编码的生物被设计成依赖合成氨基酸的生物

Alexis J Rovner¹, Adrian D Haimovich¹, Spencer R Katz¹

¹1] Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA [2] Systems Biology Institute, Yale University, West Haven, Connecticut 06516, USA.

|January 22, 2015

概括

转基因生物 (GMO) 可以通过使用基因组重新编码的生物 (GRO) 变得更安全. 这些工程细菌需要合成氨基酸才能生存,防止不受控制的生长和在开放环境中的水平基因转移.

科学领域:

合成生物学合成生物学
基因工程是一种基因工程.
微生物学微生物学

背景情况:

转基因生物 (GMO) 提供了工业效益,但需要强有力的生物安全措施.
现有的生物控制策略面临着诸如交叉养和遗传突变等局限性.
需要对转基因生物进行先进的基因隔离和生物控制.

研究的目的:

改造基因组重新编码的生物 (GROs) 以增强的生物控制.
建立依赖于外源供应的合成氨基酸 (sAAs) 的合成辅食性.
改善基因隔离并防止工程微生物的水平基因转移.

主要方法:

设计了一种Escherichia coli菌株,用于合成氨基酸 (sAAs) 的直角翻译系统.
利用多重自动化基因组工程,将TAG编码子引入22个必需基因.
开发了依赖sAA的辅助性菌株,具有多种基本基因修饰.

主要成果:

创建了强大的sAA依赖的GROs,在广泛培养后无法检测到的逃逸频率.
证明在环境测试中,通过交叉养来挽救工程性辅食.
与现有方法相比,在生物控制方面取得了显著的改进.

结论:

依赖合成氨基酸的基因组重新编码生物 (GROs) 提供了增强的生物控制.
这些GROs提供了基因隔离,阻碍了水平基因转移.
工程性辅助变推进了在开放系统中安全应用转基因生物的直角障碍.

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