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超分子光谱编码的微凝具有双链探针,可在高灵敏度下绝对和直接检测miRNA光

Filippo Causa¹, Anna Aliberti, Angela M Cusano

¹Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia (IIT) , Largo Barsanti e Matteucci 53, 80125 Naples, Italy.

Journal of the American Chemical Society

|January 24, 2015

概括

新的微凝能够在血清中直接,超敏感地检测微RNA (miRNA). 这种基于粒子的阵列提供了高精度和广泛的动态范围,优于癌症生物标志物分析的传统方法.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断学
纳米技术纳米技术

背景情况:

微RNA (miRNA) 检测对于诊断癌症等疾病至关重要.
目前的方法往往需要复杂的样本准备,缺乏灵敏度.
需要直接,高度敏感和准确的miRNA检测平台.

研究的目的:

开发用于直接和绝对检测微RNA的新型微凝.
为了创建一个敏感的,选择性的,和广泛的动态范围测定miRNA量化.
证明微凝对于分析血清中的癌症生物标志物的实用性.

主要方法:

用不同的光染料制造核心外微凝,用于编码和检测.
整合一个光探针用于特定的miRNA结合.
使用非重叠的辐射光谱进行多重光谱分析.
在没有放大的情况下测试微凝阵列在血清中直接检测miRNA.

主要成果:

实现了超敏感的miRNA检测,极限低至10~15M.
显示了广泛的动态范围 (10~9~10~15M) 和高选择性.
与定量逆转录聚合酶连锁反应 (qRT-PCR) 相比,显示出更高的准确性.
成功地在血清中直接进行了癌症生物标志物miRNAs的多重检测.

结论:

开发的微凝悬浮阵列为直接和绝对miRNA量化提供了一个强大的工具.
它的抗污染特性和灵敏度使得在没有样品预处理或目标放大的情况下进行分析.
这项技术在临床诊断中具有敏感和准确的生物标志物检测的巨大潜力.

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