酵母HSP60基因编码线粒体组装因子的表征
D S Reading1, R L Hallberg, A M Myers
1Department of Zoology, Iowa State University, Ames 50011.
Nature
|February 16, 1989
概括
酵母中的热冲击蛋白60 (hsp60) 是由HSP60基因编码的. 这种蛋白质有助于在线粒体中组装蛋白质复合体,这表明它在蛋白质折叠中起着保留的作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 热冲击蛋白 (hsp60) 在各个物种中都存在,并参与应激反应.
- 在细菌 (例如,Escherichia coli groEL) 和有机体 (例如,植物叶绿体,动物线粒体) 中发现hsp60同类物.
研究的目的:
- 克隆和描述核基因 (HSP60) 编码酵母 (Saccharomyces cerevisiae) 中的hsp60同类基因.
- 研究酵母hsp60在细胞过程中的功能,特别是线粒体复合体组合.
主要方法:
- 酵母HSP60基因的基因克隆和核酸序列分析.
- 使用先前识别的条件致死突变来描述HSP60基因的作用.
主要成果:
- 酵母HSP60基因被克隆和测序,揭示了与大肠杆菌groEL和叶绿体RUBISCO结合蛋白 (RBP) 的同质性.
- 鉴定出HSP60基因是导致线粒体多重体复合组合在非允许温度下出现缺陷的突变的基因位点.
结论:
- 酵母hsp60与细菌GroEL和植物/动物线粒体陪伴者相同.
- 与GroEL相关的蛋白质可能在促进蛋白质折叠和/或组装多个子单元复合物的过程中具有进化保守的功能.
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