鼠类和Drosophila家庭盒基因复合体具有组织和表达的共同特征
A Graham1, N Papalopulu, R Krumlauf
1Division of Eucaryotic Molecular Genetics, National Institute for Medical Research, London, England.
Cell
|May 5, 1989
概括
鼠标的Hox-2基因在胚胎中显示空间表达模式,反映了Drosophila. 这些发现表明,脊椎动物和节肢动物中Hox基因复合体的保存机制和共同祖先.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 荷姆博克斯 (Hox) 基因对胚胎发育至关重要,控制体位的形成.
- 小鼠的Hox-2复合体是调节前后模式的几个基因集群之一.
- 了解霍克斯基因表达和进化为保存的发育途径提供了洞察力.
研究的目的:
- 为了研究小鼠胚胎中七个Hox-2复合基因的空间表达模式.
- 为了比较小鼠Hox-2复合物的组织和表达与Drosophila同源基因.
- 探索脊椎动物和节肢动物中的霍克斯基因复合体的进化起源.
主要方法:
- 在现场杂交被用来分析小鼠胚胎中的基因表达.
- 在小鼠的Hox-2基因和其他homeobox基因之间进行了序列比较.
- 分析的重点是集群中的基因位置和沿前后轴的表达域.
主要成果:
- 霍克斯-2基因在中枢神经系统,外周神经系统和中皮衍生物中表现出差异性表达.
- 基因表达边界沿霍克斯-2星团逐渐向前移动,与前后位置信息相关.
- 序列和表达模式的比较揭示了小鼠Hox-2基因和Drosophila同源基因之间的明显关系.
结论:
- 在集群中Hox-2基因的位置决定了它们沿胚胎轴的表达域.
- 霍克斯基因复合体的保存组织和功能表明它们具有共同的进化起源.
- 这些发现支持了霍克斯复合体在关节动物和脊椎动物分化之前从一个共同的祖先基因进化而来的假设.
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