循环素合成驱动了早期胚胎细胞周期的过程
1Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143-0448.
Nature
|May 25, 1989
概括
青蛋提取物在体外复制细胞周期. 添加环林信使RNA (mRNA) 驱动多个细胞周期,环林蛋白的积累和降解调节了这一过程.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 细胞循环的进展是真核生物生命的基础.
- 了解控制细胞分裂的分子机制至关重要.
- 青蛋提取物为研究细胞循环调节 in vitro提供了一个强大的系统.
研究的目的:
- 在实验室中研究细胞循环进展所需的基本成分.
- 确定环林信使RNA (mRNA) 在驱动细胞周期中的作用.
- 描述细胞循环期间环林蛋白合成和降解的动态.
主要方法:
- 从Xenopus laevis (青) 蛋中制备无细胞提取物.
- 内源信使RNA (mRNA) 的耗尽,以在介相中捕获提取物.
- 添加外源环林mRNA以重新启动细胞循环.
- 在多个细胞周期中监测循环蛋白水平.
主要成果:
- 青蛋提取物能够在无细胞系统中执行多个同步的细胞周期.
- 移除内源mRNA会在相间阶段停止细胞循环.
- 外源环林mRNA的添加足以恢复和驱动多轮细胞分裂.
- 新合成的环林蛋白在间相中积聚,并在甲相-亚相过渡 (线粒分裂) 时特别降解.
结论:
- 循环素的合成和降解是关键的,受调节的事件驱动细胞循环的进展.
- 循环mRNA是启动和维持细胞周期的关键决定因素.
- 青蛋提取物作为一个有价值的模型解剖细胞分裂的分子控制.
相关概念视频
Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
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M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...


