通过Drosophila Ubx和abd-A蛋白的基因激活和DNA结合
M L Samson1, L Jackson-Grusby, R Brent
1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Cell
|June 16, 1989
概括
乌布克斯和abd-A蛋白对于虫的发育至关重要. 它们的同居域对于结合DNA是必不可少的,但对于二分化,核进入或激活,当DNA结合被另一种蛋白质促进时,它们是必不可少的.
科学领域:
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
- 德洛索菲拉 (Drosophila melanogaster) 的研究研究
背景情况:
- Ubx和abd-A基因产物对于Drosophila的胸部和腹部结构的正确发展至关重要.
- 了解这些转录因子的精确分子机制是解读发育途径的关键.
研究的目的:
- 研究本地主体在Ubx和abd-A蛋白质在DNA结合和基因激活方面的功能作用.
- 阐明对通过Ubx和abd-A.介导的二元化,核定位和转录调节的要求.
主要方法:
- 在酵母中LexA-Ubx和LexA-abdA融合蛋白的表达.
- 利用与LexA运营者或Ubx结合位点的目标基因来评估基因激活.
- 采用突变的融合蛋白来剖析家庭主体的功能.
主要成果:
- LexA-Ubx 和 LexA-abdA 融合蛋白激活了目标基因表达.
- 家庭主域对于蛋白质二分化或核进入并非必不可少.
- 当DNA结合通过LexA部分进行调解时,家庭主体对于基因激活是不可或缺的.
- 家庭主体是绑定Ubx位点所必需的,但其DNA相互作用与细菌螺旋转蛋白不同.
结论:
- 家庭主体对Ubx和abd-A与特定DNA位点的结合至关重要.
- Ubx和abd-A的基因激活主要是由DNA结合驱动的.
- 这些蛋白质的负基因调节可能涉及与其他Drosophila基因产品的相互作用.
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