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垂直传播的便IgA水平决定了染色体外的表型变异
Clara Moon1, Megan T Baldridge1, Meghan A Wallace1
1Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Nature
|February 18, 2015
概括
小鼠中的微生物差异导致显著的表型变异,模仿遗传突变. 便免疫球蛋白-A (IgA) 水平可以表明这种变异性,这表明共住或移植是为了一致的研究.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 遗传学 是一个遗传学.
背景情况:
- 转基因小鼠模型表现出表型变异,通常归因于微生物群的差异.
- 控制变异性的现有方法包括子期控制或具有定义微生物联盟的 gnotobiotic 模型.
研究的目的:
- 研究微生物因素的作用,特别是便免疫球蛋白-A (IgA) 水平,在常规养小鼠中引起表型变异的作用.
- 确定微生物变异性的标记物,并提出减轻其对实验结果影响的方法.
主要方法:
- 在不同设施的野生类型小鼠中比较便IgA水平.
- 在具有高和低IgA水平的小鼠之间进行了同居和便微生物群移植实验.
- 对IgA及其分泌成分的细菌降解的分析.
主要成果:
- 在同一设施内观察到两种便IgA水平,模仿染色体突变效应.
- 来自低IgA小鼠的细菌通过共住或移植降低了高IgA小鼠的IgA水平.
- 低IgA的小鼠在受伤反应中表现出增加的,可转移的损伤,与IgA差异有关.
- 来自IgA低的小鼠的细菌被发现会降解分泌IgA和IgA本身.
结论:
- 由于微生物因素导致的非染色体遗传变异必须在小鼠研究中考虑.
- 便IgA作为微生物变异性的标志物.
- 共同住房和便移植可以帮助标准化使用不同母体小鼠的实验.
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