由于细胞质域异质性,Fc受体异型表现出不同的涂层坑定位能力,这是细胞质域异质性的结果
H M Miettinen1, J K Rose, I Mellman
1Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510.
Cell
|July 28, 1989
概括
小鼠的巨细胞和淋巴细胞对IgG有不同的Fc受体. 巨细胞Fc受体 (FcRII-B2) 中的一个特定的细胞质尾域对于有效的内部化至关重要,与淋巴细胞异型 (FcRII-B1) 不同.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 巨细胞和淋巴细胞表达IgG Fc受体的不同异型.
- 这些异构体,FcRII-B1 (淋巴细胞) 和FcRII-B2 (巨细胞),由于替代的mRNA拼接而有所不同.
- FcRII-B1的细胞质尾巴比FcRII-B2 (47个氨基酸) 长 (94个氨基酸).
研究的目的:
- 研究FcRII-B1和FcRII-B2.2之间的细胞质域变异的功能意义.
- 确定FcRII-B1的延长细胞质尾巴如何影响Fc受体功能.
主要方法:
- 两个Fc受体异型 (FcRII-B1和FcRII-B2) 在受体阴性纤维细胞中的表达.
- 分析由每个异构体介导的连接体内化和 lysosomal 传递.
- 在克拉林涂层坑中评估受体局部.
主要成果:
- 通过FcRII-B2调解有效的联体内化和溶酶体输送.
- 通过FcRII-B1和一个减去尾巴的突变体的内细胞形成显著低效.
- FcRII-B1和尾部减去的突变体未能在涂有克拉的坑中积聚.
结论:
- 细胞质尾部的FcRII-B2包含一个域,是必要的积累在克拉斯林涂层坑.
- 这一关键域被FcRII-B1异型中存在的47个氨基酸插入所破坏.
- 细胞质尾部结构决定了Fc受体的定位和内细胞效率.
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