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在转基因小鼠中,由Homeobox基因Hox-1.1的异位表达引起的面异常

R Balling¹, G Mutter, P Gruss

¹Department of Molecular Cell Biology Max Planck Institute of Biophysical Chemistry Göttingen, Federal Republic of Germany.

|July 28, 1989

概括

在小鼠中,Hox-1.1基因的宫外表达导致严重的面缺陷和早期死亡,类似于视网膜酸暴露. 这表明在发育异常中存在一种共同的机制.

科学领域:

发展生物学发展生物学
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

鼠类Hox-1.1基因对胚胎发育至关重要,表现出精确的时间和空间表达模式.
在编排复杂的发育过程中,Homeobox基因起着至关重要的作用.

研究的目的:

在转基因小鼠中研究异位表达Hox-1.1基因的发育后果.
探索Hox-1.1错误表达和视网膜酸胚胎病变之间的潜在共享致病机制.

主要方法:

转基因小鼠的生成具有无处不在的Hox-1.1表达,由的β-actin促进剂驱动.
转基因后代的表型分析,重点关注面发育和生存率.

主要成果:

无处不在的Hox-1.1表达导致转基因小鼠的围产死亡.
观察到显著的面异常,包括口腔裂,出生时开放的眼睛和未关闭的.
观察到的表型与网膜酸诱导的胚胎病变非常相似.

结论:

宫外Hox-1.1表达破坏了正常的胚胎发生,导致严重的发育缺陷.
共享的表型表明,Hox-1.1错误表达和在发育过程中的视网膜酸毒性之间存在着共同的潜在分子途径.

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