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tctex-1:对老鼠的候选基因家族t复杂无菌位点

E Lader¹, H S Ha, M O'Neill

¹Department of Zoology, University of Texas, Austin 78712.

|September 8, 1989

概括

研究人员确定了tctex-1基因家族,这对男性生殖细胞发育至关重要. 这种基因在同卵性动物中的异常表达表明它在男性不孕症中的作用.

科学领域:

生殖生物学生殖生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

鼠标t复合体对于男性生殖细胞的发育和功能至关重要.
在t复合体中的突变可以导致变化的精子传播比率或不育.

研究的目的:

识别与t复合体相关的参与男性不育的基因.
研究tctex-1基因家族在生殖细胞发育中的作用.

主要方法:

查一个丸细胞cDNA库使用减去的cDNA探针.
在小鼠生殖细胞中分析基因表达模式.

主要成果:

多基因家族的分离,tctex-1.
tctex-1产生了丰富的,生殖细胞特异性的转录.
这种转录在同卵性动物中表达过度8倍.
异常的tctex-1表达取决于t类型的基因,并且特定于生殖细胞.

结论:

tctex-1基因家族是参与男性不孕症的有力候选者.
它的特定表达模式和同胞卵中的改变水平突出了它的功能意义.

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