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作为黄金纳米棒的生物功能连接体的甘氨酸:稳定性和向性在富含蛋白质的介质中

Isabel García^1,2, Ana Sánchez-Iglesias¹, Malou Henriksen-Lacey¹

¹†CIC biomaGUNE, Paseo de Miramón 182, 20009 Donostia-San Sebastián, Spain.

Journal of the American Chemical Society

|February 24, 2015

概括

低分子量甘氨酸有效地稳定生物流体中的等离子纳米粒子 (NP),性能优于聚乙烯甘醇 (PEG). 覆盖着甘氨酸的NP抵抗蛋白质吸附和细胞吸收,同时保持诊断和治疗应用的标选择性.

科学领域:

纳米技术纳米技术
生物材料科学生物材料科学
表面化学表面化学

背景情况:

聚乙烯甘醇 (PEG) 是稳定生物流体中的等离子纳米粒子 (NP) 的标准,防止聚合和蛋白质相互作用.
在蛋白质丰富的环境中,针对金纳米颗粒 (Au NPs) 的接体应用仍然具有挑战性.
在生物医学应用中,开发可替代稳定剂来维持NP在体内的功能至关重要.

研究的目的:

评估低分子量甘氨酸在生理条件下作为等离子体纳米粒子 (NP) 稳定剂的有效性.
为了比较基于甘氨酸的NP稳定与已建立的聚乙烯甘醇 (PEG) 方法.
评估覆盖糖的NP能够抵抗蛋白质吸附和细胞吸收,同时保持目标特异性的能力.

主要方法:

用低分子量甘氨酸涂层的球形和棒状等离子体纳米颗粒的合成和表征.
在生理条件下,基甘涂层NP与聚乙烯糖醇 (PEG) 涂层NP进行基准测试.
从含有血清的介质中评估蛋白质吸附,使用UV-Vis光谱和动态光散射 (DLS) 等技术.
评估细胞相互作用,特别是巨类细胞的细胞化.
在富含蛋白质的介质中测试糖涂层NP对碳水化合物结合蛋白的选择性.

主要成果:

低分子量甘氨酸为球形和棒状的等离子纳米颗粒提供有效的固体稳定,与聚乙烯甘醇 (PEG) 相似.
涂上糖甘的纳米颗粒对来自含血清介质的非特异性蛋白质吸附有抗性.
与未涂层或PEG涂层NP相比,巨类细胞对涂层纳米颗粒的化细胞显著减少.
糖甘表面保留了与碳水化合物结合蛋白的特定结合相互作用,即使在复杂的生物介质中.

结论:

低分子量甘氨酸为聚乙烯甘醇 (PEG) 提供了可行的替代品,用于稳定生物环境中的等离子纳米粒子.
涂上糖的纳米颗粒提供了增强的隐形特性,减少不必要的蛋白质相互作用和细胞吸收.
这些装饰着甘的纳米工具保持了特定的准能力,为先进的治疗学应用铺平了道路.

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