序列特异性,通过库库比特[8]诱导uril折叠和包含邻近侧链的纳米分子结合
Lauren C Smith1, David G Leach1, Brittney E Blaylock1
1Department of Chemistry, Trinity University, San Antonio, Texas 78212, United States.
Journal of the American Chemical Society
|February 25, 2015
概括
合成受体库库比特[8]uril识别了具有纳米分子亲和力的三. 这一突破为生物化学和生物技术应用提供了一个新的工具.
科学领域:
- 超分子化学 超分子化学
- 生物化学 生物化学
- 分子识别分子识别
背景情况:
- 已知黄[8]uril (Q8) 能与N端芳香残留物结合.
- 在合成受体中获得类似抗体的亲和力和特异性一直是长期以来的一个挑战.
研究的目的:
- 为了探索甲酸[8]uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.uril.
- 研究一种用于增强体识别的新型结合基因.
主要方法:
- 图书馆对105种的选,使用四甲基博 (MBBI) 作为光指标.
- 使用 (1) H NMR光谱学,电喷离子化质谱学和异热定位热量计进行了表征.
主要成果:
- 发现了一种新的结合模式,N端Tyr和相邻的残留物在Q8腔内结合.
- 与其同位素Tyr-Ala-Leu (Kd = 34 μM) 相比,对Tyr-Leu-Ala (Kd = 7.2 nM) 的高亲和力和特异性.
- MBBI 作为结的关闭和启动传感器.
结论:
- 黄瓜[8]uril对特定的三具有特殊的分子识别能力.
- 发现的结合基因和高亲缘关系为生物化学和生物技术应用提供了机会.
- 该系统的稳定性,成本效益和与重组蛋白质的兼容性提高了其实际效用.
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