在小鼠后脑中含有homoeobox基因的细分特异表达
P Murphy1, D R Davidson, R E Hill
1MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Nature
|September 14, 1989
概括
细分组织了胚胎的发育. 在小鼠中,Hox-2.9基因在后脑中表现出细分特异性表达,揭示了它在神经发育和细分认同中的作用.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 神经科学是一个神经科学.
背景情况:
- 胚胎细分组织身体的计划沿着前后轴.
- 虽然在Drosophila中进行了研究,但脊椎动物,特别是中枢神经系统的细分基因表达仍然不太了解.
- 脊椎动物后脑神经元,就像罗姆体一样,正在成为显著的细分单元.
研究的目的:
- 为了研究发育中的小鼠后脑中含有homoeobox基因的胚胎表达模式.
- 为了确定任何homoeobox基因是否在脊椎动物后脑发育中表现出细分特异性表达.
- 为了将基因表达与已知的细分单元 (如染色体) 相关联.
主要方法:
- 在现场杂交以检测在发育中的小鼠胚胎中的基因表达模式.
- 对小鼠homoeobox基因Hox-2.9.9表达域的分析.
- 将Hox-2.9的表达与后脑中的Krox-20的表达模式进行比较.
主要成果:
- 小鼠homoeobox基因Hox-2.9在发育中的后脑中以特定的细分方式表达.
- 霍克斯-2.9的表达精确地位于单个神经元体内,即罗姆波体4.
- Hox-2.9的表达域位于Krox-20基因的表达区域之间.
结论:
- 霍克斯-2.9在成长中的小鼠后脑的细分组织中起作用.
- 这一发现为脊椎动物神经发育中homoeobox基因的细分特异表达提供了证据.
- 霍克斯-2.9在4号染色体内的精确定位突显了它在后脑区分标识的定义中的参与.
相关概念视频
Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
Determination
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...


