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害虫防治害虫控制害虫防治害虫防治害虫防治通过塑体中长长的双链RNA的表达,对昆虫害虫进行全面的作物保护

Jiang Zhang¹, Sher Afzal Khan², Claudia Hasse¹

¹Max-Planck-Institut für Molekulare Pflanzenphysiologie, D-14476 Potsdam-Golm, Germany.

Science (New York, N.Y.)

|February 28, 2015

概括

在质体中产生的长双链RNAs (dsRNAs) 可以保护作物免受害虫的侵害. 这种新的RNA干扰 (RNAi) 策略为可持续农业提供了无农药的解决方案.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
农业科学农业科学

背景情况:

使用双链RNAs (dsRNAs) 的RNA干扰 (RNAi) 是一种有效的害虫控制方法.
植物RNAi通路可以降解dRNAs,限制它们在作物保护中的有效性.
由于缺乏RNAi机制的质体,它们为dsRNA生产提供了潜在的庇护所.

研究的目的:

为了研究叶绿体在植物中稳定dSRNA生产的潜力.
开发一种基于RNAi的新型作物保护策略,使用叶绿体表达.
评估dSRNA针对科罗拉多马虫的基本基因的有效性.

主要方法:

从质体基因组产生表达长dRNA的转质体土豆植物.
在质体内量化dSRNA积累.
评估植物保护对科罗拉多马甲虫的草食和幼虫生存.

主要成果:

长dsRNAs稳定地产生并积累到显著水平 (0.4%的总细胞RNA) 在质体.
转塑性土豆植物显示出对科罗拉多土豆虫草食的保护.
食dRNA生产植物的幼虫表现出致命的影响.

结论:

叶绿体表达是一种可行的策略,用于在植物中产生功能性长dRNAs.
这种方法可以有效地,无农药地保护作物免受昆虫害虫的侵害.
使用叶绿体绕过了植物RNAi的降解,提高了dsRNA的稳定性和有效性.

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