进化. 进化. 进化的. 通过重新连接调节系统,鞭毛运动的进化复活
Tiffany B Taylor1, Geraldine Mulley1, Alexander H Dills2
1School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK.
概括
细菌通过重新连接基因调节网络,迅速发展出运动性. 调节基因NtrC的突变允许不运动的Pseudomonas光体通过两步进化过程重新获得鞭毛.
科学领域:
- 进化生物学是进化的生物学.
- 微生物遗传学微生物遗传学
- 分子生物学分子生物学
背景情况:
- 基因调节网络是进化的核心.
- 不动的Pseudomonas fluorescens菌株是通过删除fleQ基因进行的.
研究的目的:
- 为了研究 Pseudomonas fluorescens 中 flagella 恢复的进化途径.
- 了解自然选择如何重新连接基因调节网络.
主要方法:
- 工程化不运动的细菌菌株 (Pseudomonas fluorescens 缺乏 fleQ).
- 应用了强大的运动性选择.
- 在96小时内分析了进化步骤和基因突变.
主要成果:
- 细菌在96小时内通过可重复的两步进化途径恢复了鞭毛.
- 第一步:突变增加了化NtrC,劫持了fleQ regulon并破坏了代谢.
- 第二步:NtrC中的一个功能切换突变将其重定向到鞭毛调节.
结论:
- 自然选择可以迅速重新连接基因调节网络.
- 进化途径可能涉及最小数量的可重复的突变步骤.
- 基因重复和监管网络的招募是关键的进化过程.
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