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A Visual Assay to Monitor T6SS-mediated Bacterial Competition
Published on: March 20, 2013
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第六类分泌系统的结构 收缩性外
Mikhail Kudryashev1, Ray Yu-Ruei Wang2, Maximilian Brackmann3
1Focal Area Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland; Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Cell
|February 28, 2015
概括
细菌使用可收缩的纳米机器,称为VI型分泌系统 (T6SS),将分子输入细胞. 我们确定了收缩的T6SS的原子结构,揭示了它的组装和与辅助蛋白的相互作用.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 六型分泌系统 (T6SS) 是一种复杂的纳米机器,由细菌用于细胞间传输.
- T6SS通过接触依赖的作用蛋白向点细胞传递,影响微生物群落和宿主相互作用.
- 了解T6SS收缩的结构基础对于破译其作用机制至关重要.
研究的目的:
- 为了确定受感染的Vibrio cholerae T6SS的原子分辨率结构.
- 阐明T6SS外组装和收缩背后的分子机制.
- 为了研究T6SS外与辅助ATPase ClpV.之间的相互作用.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来获得合同的T6SS外的原子分辨率结构.
- 生物信息分析和结构比较被用来理解子单元相互作用和保存的折叠.
- 生物化学试验被用来研究ClpV在膜动态中的作用.
主要成果:
- 受感染的Vibrio cholerae T6SS外由VipA和VipB蛋白组成,形成一个六个启动螺旋.
- 由VipA和VipB的β链组装的保存的核心域稳定了螺旋结构.
- 独特的外层结构表明与ClpV ATPase相互作用的机制,促进重复循环的效应器传递.
结论:
- 该研究提供了合同T6SS的第一个原子分辨率结构,揭示了与菌体相比保存和独特的特征.
- 这些发现为细菌纳米机器的组装和功能提供了机械的洞察力.
- 这项工作为了解T6SS如何促进大分子跨膜转移奠定了基础.
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