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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
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这种RNA结合蛋白震动调节了circRNAs的形成
Simon J Conn1, Katherine A Pillman2, John Toubia2
1Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA 5000, Australia.
Cell
|March 14, 2015
概括
循环RNAs (circRNAs) 在细胞变化过程中受到调节,如EMT. 震 (QKI) 蛋白控制circRNA的产生,表明有目的的合成和细胞过程中的特定作用.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 遗传学 是一个遗传学.
背景情况:
- 循环RNA (circRNAs) 是动物中普遍存在的非编码RNA,但它们的功能相关性仍在争论中.
- 目前尚不清楚circRNAs是否仅仅是拼接副产品,还是具有细胞作用的活性调节分子.
研究的目的:
- 为了研究在人类上皮质-介质细胞转换 (EMT) 期间circRNAs的调节.
- 确定替代拼接因子Quaking (QKI) 在circRNA生产中的作用.
主要方法:
- 在EMT期间分析circRNA表达.
- 研究震 (QKI) 蛋白水平对circRNA丰度的影响.
- 评估内部QKI结合基因在circRNA形成中的作用.
主要成果:
- 在EMT期间,数百个circRNAs被动态调节.
- 超过三分之一的丰富的circRNAs是由Quaking (QKI) 调节的,它本身在EMT期间是调节的.
- QKI结合动机对于调节的circRNA产生至关重要,它们的添加可以诱导de novocircRNA形成.
结论:
- 循环RNAs是有目的地合成的,它们的产生是由细胞类型特定的机制调节的.
- 这些发现表明,circRNAs扮演着特定的生物学角色,特别是在EMT的背景下.
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