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相关概念视频

MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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相关实验视频

Updated: Apr 15, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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N6-甲基氨酸标记了初级微RNA用于加工.

Claudio R Alarcón1, Hyeseung Lee1, Hani Goodarzi1

  • 1Laboratory of Systems Cancer Biology, Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.

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|March 25, 2015
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概括
此摘要是机器生成的。

甲基转移酶类3 (METTL3) 将m(6) A标记添加到primiRNA中,使得DGCR8识别和microRNA处理成为可能. 这种修改对于miRNA生物发生和全球miRNA水平至关重要.

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 微RNA (miRNA) 生物发生始于微处理器复合体 (DGCR8/DROSHA) 的初级miRNA (pri-miRNA) 处理.
  • 对于DGCR8对其他RNA结构上的primiRNAs识别的确切机制尚不清楚.

研究的目的:

  • 阐明DGCR8识别和结合primiRNAs的机制.
  • 确定调节miRNA生物发生的初始步骤的因素.

主要方法:

  • 细胞实验涉及METTL3耗尽和功能的增加.
  • 生物化学测定包括体外加工反应.
  • 对primiRNA和成熟miRNA水平的分析.

主要成果:

  • 在N(6) - 甲基亚丁素 (m(6) A部位上甲基化METTL3的pri-miRNAs,标记它们为DGCR8结合.
  • METTL3的枯竭减少了DGCR8-pri-miRNA的相互作用,导致成熟miRNA的减少和primiRNA水平的增加.
  • 在体外研究证实了m(6)A足够用于primiRNA处理;METTL3在全球范围内增强了miRNA成熟.

结论:

  • 由METTL3介导的m(6)A修饰是一种关键的转录后调节器,促进了DGCR8的primi-miRNA识别和处理.
  • 这一发现揭示了一种控制miRNA生物发生的新机制.