一个"智能"128Xe NMR生物传感器用于pH依赖细胞标记
Brittany A Riggle1, Yanfei Wang1, Ivan J Dmochowski1
1Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, United States.
Journal of the American Chemical Society
|April 8, 2015
概括
我们开发了一个智能 xenon-129 NMR 生物传感器,使用-加密复合体. 这种生物传感器通过改变其结构来检测酸性环境,从而使pH依赖的细胞标记成为潜在的癌症诊断.
科学领域:
- 生物物理化学 生物物理化学
- 化学生物学 化学生物学
- 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学
背景情况:
- 形变化对于生物功能至关重要.
- 开发用于酸性环境的敏感探头对于生物和医学应用很重要.
- -129NMR为分子成像和传感提供了独特的特性.
研究的目的:
- 为了创建一个"智能"的-129 NMR 生物传感器.
- 为了利用形变化用于pH依赖的细胞标记.
- 探索这种生物传感器在酸性环境和癌症诊断中的应用.
主要方法:
- 一个30mer的EALA重复与一个加密主体分子的结合.
- 根据pH值依赖的形状的表征 (pH值为5.5的α-螺旋,pH值为7.5的无序).
- 测量-129NMR化学转移和超极化化学交换和转移 (超CEST) 的NMR.
主要成果:
- -129的NMR化学转移显示出强烈的pH依赖 (Δδ = 3.4 ppm).
- 酸的检测极限低至10~11M,明显低于其他NMR pH报告器.
- 在pH 5.5的细胞膜插入诱导了大量的13.4 ppm下场转移在cryptophan-{129) Xe NMR信号.
结论:
- 开发的-129 NMR生物传感器通过形转换有效检测pH值的变化.
- 这项技术使得可以对的结构和功能进行超敏感的探测.
- 潜在的应用包括pH依赖的细胞标签用于癌症诊断和治疗监测.
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