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通过用催化剂连接抗体对共价标签进行细胞表面受体动态的分析

Takahiro Hayashi1, Yuki Yasueda1, Tomonori Tamura1

  • 1†Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan.

Journal of the American Chemical Society
|April 9, 2015
PubMed
概括

新型催化剂-抗体结合物能够精确标记活细胞上的细胞表面受体,如EGFR和HER2. 这种技术允许详细研究蛋白质动态和相互作用,克服基因工程方法的局限性.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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