先进的转录因子针对核体上的部分DNA动机,以启动重编程
Abdenour Soufi1, Meilin Fernandez Garcia1, Artur Jaroszewicz2
1Institute for Regenerative Medicine and Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Smilow Center for Translational Research, Building 421, 3400 Civic Center Boulevard, Philadelphia, PA 19104-5157, USA.
Cell
|April 21, 2015
概括
开拓者转录因子 (TF) 使用DNA结合域访问沉默的染色质,启动细胞命运变化. 它们结合核细胞和识别其表面部分DNA图案的能力驱动了这一关键过程.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 开拓性转录因子 (TFs) 对于通过访问静态染色质来启动细胞命运变化至关重要.
- 福克斯A是一种经过充分研究的先驱TF,利用翼状螺旋DNA结合域 (DBD) 来结合核细胞和紧的染色体.
- 了解其他先驱TF的机制对于理解细胞重编程至关重要.
研究的目的:
- 为了比较Oct4,Sox2,Klf4和c-Myc.的核细胞和染色体向活动.
- 研究DNA结合域 (DBDs) 在这些因子的先驱活动中的作用.
- 阐明这些因素是如何将体细胞集体重编程为多能性的.
主要方法:
- 试验室结合试验用纯化的Oct4,Sox2和Klf4蛋白进行.
- 在体内进行色素向分析.
- 对DNA结合域结构和功能的比较分析.
主要成果:
- 在实验室中,Oct4,Sox2和Klf4蛋白质表现出核酶体结合能力.
- 这些因素在体内优先准沉默的,核细胞丰富的染色体位点.
- 开拓者活动与DBD识别核细胞上部分DNA图案的能力相关.
- 因素之间的协调结合有助于部分图案的识别.
结论:
- 开拓性TFs结合核细胞和识别其表面部分DNA图案的能力是它们功能的关键.
- 这些机制提供了关于先驱因子如何访问和修改天真染色体的见解.
- 了解这些相互作用对于推进细胞重编程和再生医学至关重要.
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