分解. 分解. 微管异化引导染色体在线分裂期间
Marin Barisic1, Ricardo Silva e Sousa2, Suvranta K Tripathy2
1Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. Instituto de Investigação e Inovação em Saúde-i3S, Universidade do Porto, Portugal.
Science (New York, N.Y.)
|April 25, 2015
概括
微管异化指导着染色体聚集中的中位素相关蛋白E (CENP-E). 这种特定的翻译后修改指示CENP-E运动蛋白调整细胞中的染色体.
科学领域:
- 细胞生物学 细胞生物学
- 分子电机分子电机
- 细胞骨动力学 细胞骨动力学
背景情况:
- 染色体凝聚对于准确的细胞分裂至关重要,确保染色体在线于线性螺旋赤道.
- 中心分子关联蛋白E (CENP-E) 是一种激素电机,对于将染色体移动到细胞中心至关重要.
- 尽管微管的方向不清楚,CENP-E通过何种机制将近极染色体导航到赤道,但仍不清楚.
研究的目的:
- 研究微管子转化后修改在指导染色体凝聚过程中的CENP-E中的作用.
- 为了确定特定的微管状况是否会影响CENP-E对线粒状赤道的方向性.
主要方法:
- 利用基于细胞的测试来观察染色体运动和微管异化.
- 进行了 in vitro 用纯化的 CENP-E 和异化微管进行的溶解实验.
- 在细胞中操纵了管类型突化,以改变微管类型突化模式.
主要成果:
- 极近位染色体的染色体聚集取决于螺旋微管的特定的翻译后异位化.
- 试验室研究显示,在被催化素化微管上增强了CENP-E的运输.
- 阻断管类型突化导致广泛的微管类型突化和随机染色体运动由CENP-E.
结论:
- 在染色体凝聚过程中,微管异化作为CENP-E的关键指导线索.
- 这种修改特别指导CENP-E介导的染色体运输向线粒旋赤道.
- 这些发现阐明了一种新的调节染色体分离忠实性的机制.
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