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神经活动通过神经蛋白-3分泌促进质瘤生长

Humsa S Venkatesh¹, Tessa B Johung¹, Viola Caretti¹

¹Department of Neurology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

|April 28, 2015

概括

活跃的神经元刺激高度质瘤 (HGG) 的生长. 研究人员发现,分泌的蛋白质神经灵-3 (NLGN3) 驱动了这种HGG增殖,为脑瘤治疗提供了一个新的点.

科学领域:

神经科学是一个神经科学.
在瘤学瘤学.
分子生物学分子生物学

背景情况:

活跃的神经元影响神经前体细胞,这是高度质瘤 (HGG) 的起源.
大脑瘤微环境在HGG进展中的作用正在调查中.

研究的目的:

在体内研究神经元活动对HGG生长的影响.
识别激活神经元分泌的促进HGG增殖的特定分子.

主要方法:

在儿科质母细胞瘤异种移植模型中对神经元活动的光遗传控制.
从HGG细胞培养物的刺激皮质切片中分析条件介质.
识别和功能验证神经蛋白-3 (NLGN3) 作为一个线索基因.

主要成果:

通过光遗传刺激的神经元促进了HGG的生殖和生长.
从活跃神经元分泌的因素,特别是NLGN3,对于HGG细胞增殖来说是足够的和必要的.
NLGN3激活了PI3K-mTOR通路,并且与人类HGG患者的生存率降低相关.

结论:

神经元活动显著影响脑瘤微环境和HGG生长.
分泌的NLGN3是神经元活动驱动的HGG增殖的意外调解者.
NLGN3代表了高度质瘤的潜在治疗点.

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