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RNA Secondary Structure Prediction Using High-throughput SHAPE

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RNA 的结构.RNA 的结构. 艾滋病毒-1RNA包装信号的结构

Sarah C Keane¹, Xiao Heng¹, Kun Lu¹

¹Howard Hughes Medical Institute (HHMI) and Department of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA.

Science (New York, N.Y.)

|May 23, 2015

概括

艾滋病毒-1RNA领导者形成了一个独特的结构,控制病毒包装. 这种结构暴露了Gag蛋白结合的关键瓜诺辛,促进病毒组装和基因组选择.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
病毒学病毒学

背景情况:

人类免疫缺陷病毒1型 (HIV-1) 基因组的5'领导者具有保护的元素,这些元素对于选择性包装未连接的二维病毒RNA成为新的病毒颗粒至关重要.
了解这些元素对于理解HIV-1复制和开发抗病毒策略至关重要.

研究的目的:

确定HIV-1领导者的155核酸区的三维结构,称为核心封闭信号 (Ψ(CES)).
阐明这种RNA结构如何促进选择性病毒RNA包装,Gag蛋白结合和病毒转化调节.

主要方法:

使用 (2) H编辑的核磁共振 (NMR) 光谱方法来分析RNA结构.
研究了 Ψ ((CES) 区域的结构特征,重点是基配对和核酸可访问性.

主要成果:

艾滋病毒-1 Ψ(CES) RNA区域采用了一个意想不到的双联三向结形状.
这种结构隔离了主要接供体和翻译启动的重要部位.
关键的guanosines,关键的RNA包装和Gag蛋白相互作用,暴露在螺旋连接处.

结论:

确定的RNA结构为理解HIV-1的选择性病毒基因组包装提供了分子基础.
该结构解释了翻译衰减和增强的Gag结合的机制.
这种结构洞察对于理解病毒组装过程中未连接的二维基因组如何被选择至关重要.

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