一种植物免疫受体检测到针对WRKY转录因子的病原体效应体
Panagiotis F Sarris1, Zane Duxbury1, Sung Un Huh1
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
Cell
|May 23, 2015
概括
植物免疫受体,如核酸结合,富含白素的重复蛋白 (NB-LRR) 检测病原体. RRS1-R受体使用其融合的WRKYDNA结合域来识别细菌效应体,激活植物防御机制.
科学领域:
- 植物免疫力 植物免疫力
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 多细胞真核生物依赖细胞内免疫受体进行病原体防御.
- 植物核酸结合,富含白素的重复 (NB-LRR) 免疫受体通常具有融合的蛋白质域.
- 阿拉比多普西斯RRS1-R蛋白质是NB-LRR受体,含有C端WRKYDNA结合域,并与RPS4一起运作.
研究的目的:
- 研究植物细胞内免疫受体的监控机制.
- 了解RRS1-R/RPS4复合体如何检测细菌效应器AvrRps4和PopP2.4的作用.
- 阐明 WRKY 域在 RRS1-R 的功能和植物防御中的作用.
主要方法:
- 研究植物免疫受体和细菌效应器之间的蛋白质-蛋白质相互作用.
- 分析效应体结合对受体活性,特别是DNA结合的功能影响.
- 在WRKY蛋白家族中对效应因子标进行比较分析.
主要成果:
- RRS1-R/RPS4复合体检测到细菌效应器AvrRps4和PopP2,从而触发了防御反应.
- 无论是AvrRps4还是PopP2,都与RRS1-R.的WRKY域相互作用.
- PopP2通过乙化抑制RRS1-R的DNA结合,这表明效应因子干扰了WRKY转录因子依赖的防御.
结论:
- RPS4/RRS1复合体集成了一个"诱"域 (WRKY) 来感知针对WRKY蛋白的效应器.
- NB-LRR受体对,其中一个成员具有额外的域,可以检测针对该特定域的病原体效应物.
- 这种机制为植物如何识别操纵宿主转录因子的病原体效应因子提供了一个模型.
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