单细胞转录组分析揭示了激活休眠神经干细胞的信号
Yuping Luo1, Volkan Coskun2, Aibing Liang3
1Stem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China; College of Life Sciences, Nanchang University, Nanchang 330031, China.
Cell
|May 23, 2015
概括
在成年大脑中识别了休眠的内膜神经干细胞 (NSC). 血管内皮生长因子 (VEGF) 和基本纤维细胞生长因子 (bFGF) 激活这些NSC,促进神经谱系的分化和迁移,提供新的治疗途径.
科学领域:
- 神经科学是一个神经科学.
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
背景情况:
- 鉴定特定组织干细胞的特征是具有挑战性的,因为它们的稀缺性和复杂的微环境.
- 成年小鼠前脑神经性区域中的附体细胞是神经干细胞 (NSC) 的潜在来源.
研究的目的:
- 在成年小鼠前脑中分子表征静止表皮细胞 (CD133(+) /GFAP(-).
- 为了研究表原性NSCs的激活和分化潜力.
主要方法:
- 单细胞转录组分析.
- 权重基因共同表达网络分析 (WGCNA).
- 在体内给予血管内皮生长因子 (VEGF) 和基本纤维细胞生长因子 (bFGF).
主要成果:
- 静止的附带性CD133 ((+) /GFAP ((-) 细胞拥有独特的基因网络,富含免疫反应和血管原因子受体基因.
- 施用VEGF激活了整个侧腔和第四心室内的内膜性NSCs.
- 同时使用VEGF和bFGF诱导神经谱系的分化和迁移.
结论:
- 休眠的上垂体NSC存在于整个中枢神经系统 (CNS) 室内表面.
- 免疫和血管生成信号,特别是VEGF,可以激活这些休眠的NSC,特别是在受伤后.
- 这一发现为大脑修复和再生策略开辟了新的可能性.
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