人类产前生殖系中的DNA脱甲基化动力学
Sofia Gkountela1, Kelvin X Zhang2, Tiasha A Shafiq1
1Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Cell
|May 26, 2015
概括
人类生殖细胞表现出独特的DNA甲基化模式,与干细胞不同. 这些细胞在全球DNA脱甲基化事件后作为研究表观基因组的自然模型.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 全球DNA脱甲基化对人类早期发育和干细胞重编程至关重要.
- 在人类生殖细胞中,DNA甲基化重编程的确切程度在很大程度上仍未被描述.
研究的目的:
- 为了研究人类产前生殖细胞的DNA甲基组和转录组.
- 为了比较人类生殖系表观遗传与多能干细胞 (PSC) 和内部细胞质量 (ICM).
- 确定在生殖线和ICM中受脱甲基化保护的区域.
主要方法:
- 全基因组双硫酸盐测序 (WGBS) 和RNA测序 (RNA-seq) 在人类产前生殖细胞 (53-137日发育) 上进行.
主要成果:
- 与人类PSC和胚胎细胞ICM相比,人类的生殖系表现出明显的转录组和甲基组.
- 特定的超低甲基化"热点"在转子体中被确定,这些转子体在生殖细胞和ICM细胞中受到脱甲基化保护.
- 人类生殖细胞提供了一个体内系统,可以观察全球DNA脱甲基化后的表观基因组变化.
结论:
- 人类生殖系甲基组是独一无二的,并且与其他多能细胞类型有很大不同.
- 生殖线和ICM中的可移植元素具有受保护的甲基化状态.
- 人类生殖系作为一个有价值的自然模型,用于研究经过脱甲基化后的表观基因组动力学.
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